Howard C. Jordi, Ph.D.
EDUCATION:
- 1/74 Ph.D. from Northern Illinois University, DeKalb, Illinois.
- 9/67 – 7/73 Graduate student in Biochemistry, Northern Illinois University, DeKalb, Illinois.
- 9/64 – 8/67 B.S. with high scholastic honors, Northern Illinois University, DeKalb, Illinois. Major in Chemistry with a minor in Math.
- 9/63 – 6/64 Undergraduate Elgin Community College, Elgin, Illinois. Major in Chemistry.
- 9/60 – 6/63 Elgin High School, Elgin, Illinois.
WORK HISTORY:
12/2008 to Present
Founder and Director of Research and Development at Jordi Labs LLC. Since December 2008, he has directed the development of several new chromatographic resins. He is heavily involved in additional product development work and also in the analytical service division, providing consultation and guidance in everyday operations. (see below for more information)
10/1980 to 12/2008
Founder, President and CEO of Jordi Labs LLC, an independent analytical testing laboratory specializing in chromatographic separations and column development. He has led the development of several types of GPC and HPLC columns. Additional product development work is ongoing. He is now developing several new product lines which include divinylbenzene based reverse phase HPLC packings, a solid particle non porous resin for rapid protein assays, sulfonated divinylbenzene based gels for carbohydrate and organic acid analyses, glucose derivatized divinylbenzene gels for normal phase HPLC and aqueous GPC work, etc. Additionally, he is researching the development of radically new column chemistries such as polyamide gels for GPC work. Finally, he has performed extensive GPC/HPLC work on polyolefins and additives analyses as well as deformulations of various polymeric systems using techniques such as GC, HPLC, GPC/SEC, proton and carbon NMR, IR, DSC, TGA, TMA, ESCA, EDS, ICP, PYMS, GCMS, LCMS, LCFTIR, etc.
2/1980 to 9/1980
Employed by LC Laboratories of Newton, Massachusetts as Senior Chemist. His chief responsibilities were to set up and manage a complete polymer GPC program, both room temperature and high temperature, to include special methods such as aqueous GPC and LiBr/DMF work etc. Additionally, he began a liquid chromatography column repacking business and was solely responsible for developing this line of our work. Finally, he supported other chemists by developing the most efficient LC methods possible, which were then applied to the company’s prep LC work.
4/1978 to 2/1980
Employed by Waters Associates of Milford, Massachusetts as Chemicals Applications Manager for the Chromatography Supplies Division. The position included responsibility for applications development on their complete chemical product line, both current and anticipated. The work centered on the development of a post column detector for use in free amino acid analysis and on development of the chromatographic systems required. Additionally, extensive interaction with their R&D Department was required in attempts to improve product reliability and performance. For example, process development work was performed to prevent HPLC column voiding and improve both resolution and reproducibility. Additional tasks were the development of carbohydrate, protein, and lipid separation systems.
6/1977 to 4/1978
Employed by Waters Associates as Laboratory Manager for the Life Science Division. A variety of liquid chromatographic methods were developed in order to support the field sales force with individual customers. The methods developed included, but were not limited to, steroids, both free and derivatized, fatty acids, phospholipids, proteins, peptides, carbohydrates, and nucleic acids. This position also included giving seminars and lectures on lipid chromatography, as well as teaching in the liquid chromatography school. Additionally, this position involved running a sales oriented workshop program in which his lab team would go to a different city each month, set up shop, and attempt to separate any and all samples brought to the team by potential customers.
1/1977 to 6/1977
Employed by Waters Associates as a Biological Applications Chemist under the direction of Dr. Gerald L. Hawk. A variety of liquid chromatographic separations were developed to the feasibility stage with specialties in lipid and carbohydrate analyses. Additionally, some work was done toward the high performance liquid chromatographic separation of proteins. The work also included giving seminars and lectures on liquid chromatography both in-house and on the road, as well as teaching in the liquid chromatography school. Finally, his responsibilities included the development of biological preparative separations with the Prep LC/System 500 liquid chromatograph.
8/1973 to 1/1977
Employed by the U.S. Army at the United States Army Institute of Dental Research under the direction of Dr. Marvin F. Grower. A variety of techniques, including high performance liquid chromatography, fluorescence, spectrophotometric, and chemical methods were used to characterize various drugs contained in biodegradable copolymers of polylactic and polyglycolic acid. High performance liquid chromatographic separations of copolymer and various drugs on uStyragel succeeded in yielding the molecular weight distribution of the polymer and the purification of the drug in a single run. Other studies involved measuring the enzymatic degradation of dental plaque using a sensitive fluorescence assay and the purification and separation of fatty acids using a high performance liquid chromatographic method. The fatty acids were derivatized to make them good absorbers of ultraviolet radiation and were separated by reverse-phase chromatography. The work also involved some teaching of biochemistry in the masters level program offered. The program was associated with the George
Washington University and he was appointed as an Assistant Professorial lecturer from 1974 through 1977.
1/1970 to 1/1973
Graduate thesis research under the direction of Professor J.E. Erman. This work involved kinetic studies of the cytochrome c peroxidase mediated oxidation of ferrocyanide by hydrogen peroxide. Both transient state and steady state kinetics were studied as a function of pH and ionic strength in order to determine the nature of the charge on the active site of the enzyme. The transient state work was done using stopped flow methods, while the bulk of the steady state studies were run on a Cary 14 spectrophotometer. Other work included the isolation and purification of cytochrome c peroxidase from Brewer’s yeast and computer aided analysis of the huge amount of kinetic data gathered from the above mentioned studies.
6/1967 to 12/1969
Undergraduate and graduate research, under the direction of Professor R.J. Albers, on the formation and characterization of polypeptides using a variety of methods including nuclear magnetic resonance, optical rotatory dispersion, circular dichroism, and ultracentrifugation in an attempt to correlate amino acid sequence with tertiary structure.
SOCIETIES:
- Society of Plastics Engineers
- American Chemical Society
- The American Institute of Chemists
AWARDS:
- National Science Foundation Graduate Traineeship, 1967-1971
- Research Assistantship at Northern Illinois University, 1971-1973
PUBLICATIONS:
(1) Jordi, H.C., and Erman, J.E., Cytochrome c Peroxidase Catalyzed Oxidation of Ferrocyanide by Hydrogen Peroxide. Transient State Kinetics. Biochemistry, 13, 3734-3740, 1974.
(2) Jordi, H.C., and Erman, J.E., Cytochrome c Peroxidase Catalyzed Oxidation of Ferrocyanide by Hydrogen Peroxide. Steady State Kinetics. Biochemistry, 13, 3741-3754, 1974.
(3) Jordi, H.C., Grower, M.F., and Kramer, G., Effects of Glucanase, Mutanase, and Protease on Pooled Dental Plaque. Journal of Dental Research, 54, 60, Special Issue A. 1975.
(4) Jordi, H.C., Grower, M.F., and Curtight, D.C., The use of Biodegradable Polymers for Slow Drug Release. Journal of Dental Research, 55, 61, Special Issue B, 1976.
(5) Takayama, K., Qureshi, N., Jordi, H.C., and Schnoes, H.K., Separation of a Homologous Series of Mycolic Acids from Mycobacterium Tuberculosis H37Ra by High Performance Liquid Chromatography, Liquid Chromatography Symposium I: Biological/Biomedical Applications of Liquid Chromatography, Hawk, G.L., Ed., Marcel Dekker, Inc., New York, pp. 91-101, 1979.
(6) Jordi, H.C., Separation of Long and Short Chain Fatty Acids as Naphthacyl and Substituted Phenacyl Esters by High Performance Liquid Chromatography, Journal of Liquid Chromatography, 1, 215-230, 1978.
(7) Qureshi, N., Takayama, K., Jordi, H.C., and Schnoes, H.K., Characterization of the Purified Components of a New Homologous Series of Mycolic Acids from Mycobacterium Tuberculosis H37Ra, Journal of Biological Chemistry, 253, 5411-5417, 1978.
(8) Takayama, K., Qureshi, N., Jordi, H.C., and Schnoes, H.K., Purification of Mono and Diunsaturated C22-C47 Fatty acids from Mycobacterium Tuberculosis H37Ra as Their p-Bromophenacyl Esters by High Performance Liquid Chromatography, Liquid Chromatography Symposium II: Biological/Biomedical Applications of Liquid Chromatography, Hawk, G.L., Ed., Marcel Dekker, Inc., New York, pp. 375-394, 1979.
(9) Miller, R.A., Bussell, N.E., Ricketts, C.K., and Jordi, H.C., Analysis and Purification of Eugenol, Journal of Dental Research, 58, 1394-1400, 1979.
(10) Takayama, K., Jordi, H.C., and Benson, F., Separation of Fatty Acids as Their p-Bromophenacyl Esters on a C30-Bonded Silica Column by High Performance Liquid Chromatography, Journal of Liquid Chromatography, 3, 61-69, 1980.
(11) Takayama, K., Qureshi, N., Jordi, H.C., and Schnoes, H.K., Recycling HPLC of p-Bromophenacyl Esters of Saturated C35-56 Fatty Acids from Mycobacterium Tuberculosis H37Ra on a Silica Column, Journal of Liquid Chromatography, 2, 861-873, 1979.
(12) Jordi, H.C., Neue, U.D., Quinn, H.M., and Rausch, C.W., Study of Radially Compressed Columns; Chromatographic Theory and Applications on Selected Lipids, Liquid Chromatography Symposium III: Biological/Biomedical Applications of Liquid Chromatography, Hawk, G.L., Marcel Dekker, Inc., New York, pp. 327-346, 1981.
(13) Schlager, S.I., and Jordi, H.C., Separation of Cellular Phospholipid, Neutral Lipid, and Cholesterol by HPLC, BBA, 665,355-358, 1981.
(14) Lotfy, P., Jordi, H.C., and Bruno, J., Determination of Vitamin D in Multivitamin Preparations by HPLC, Journal of Liquid Chromatography, 4, 155-164, 1981.
(15) Doos, W.GH., duMoulin, G.C., Jordi, H.C., Orf, H.W., Seder, R., Skea, W., and Sullinger, J., Field Tests on Four State-of-the-Art Blood Chemistry Analyzers in a Mobile Laboratory Setting, Military Medicine, 153, 81-86, 1988.
(16) Doos, W.G., Jordi, H., Fisher, S., and Ambraziejus, R., Developing a Functional Mobile Laboratory, Proceedings of SAFMLS, 17, Slide Presentation, 1988.
(17) Jordi, H.C., Peers, S., Duncan, S., Howley, J., Hartwell, R., Eggelston, A., Stephens, D., Development of a Meaningful Training Program in Toxicolory Using Techniques such as High Performance Liquid Chromatography, Gas Chromatography, Infrared and UV-VIS Spectroscopy, Proceedings of SAFMLS, 17, Slide Presentation, 1988.
(18) Jordi, H.C., Savage, W. and Bichard, F., Microwave Extraction of a Range of Additives Including Some Hindered Amine Light Stabilizers From Polyolefin Materials, Pittcon Symposium, Paper 1209, (1995).
(19) Chapter in Column Handbook for Size Exclusion Chromatograpy, Academic Press, Chi-San Wu Ed. etc., 1999.