Tips for Best Results with GPC Columns & Gel DVB Columns
1. Run your column at 0.5-2.0mL/min for maximum life and best data. We usually recommend a flow rate of 1.2mL/min.
2. For use in TCB at 140-150°C we purge the columns at 0.2mL/min overnight with TCB at 40°C and then ramp up to temperatures over 300min.
3. If you notice a calibration change after significant use, you may need a clean frit{s} particularly on the column inlet. The original inlet frit is 5µ; and, if it clogs, it will contribute to shearing of high MW polymer and thus, must be changed.
4. For special solvents i.e. DMSO/H2O, MeOH, Acetone, please call us first.
5. For any solvent changeover involving miscible solvents, it is best to purge with the new solvent at 0.2mL/min over night. Immiscible solvents require an intermediary solvent that both the initial and final solvent are miscible with.
6. For Mixed-Bed, 104Å and 105Å. materials never exceed 2000psig as this will crush the gel. For solid bead, 100Å, 300Å, 500Å and 1000 Å gels you may run up to 8000psig with no problems.
7. If you have any specific questions, please call us.