Liquid Chromatography Mass Spectroscopy (Quantitative)

The sample is dissolved into a suitable solvent and then separated by liquid chromatography. Sample components are then passed into an electrospray ionization source for analysis by mass spectrometry. Comparison against reference standard(s) of known concentration often allows for compound quantitation. This technique can be applied successfully in some cases in which non-volatility of sample components prevents their detection by other analytical techniques.