Homogeneity is one of the most basic and important protein properties in general life science and biopharmaceutical applications. In an ideal study, the protein of interest would exist throughout the sample in a consistent form and size to ensure optimal purity for accurate characterization. This enables chemists to precisely identify protein properties for specific downstream applications. Yet proteins are unlikely to exist in this ideal state for long, given the sheer variety of potential aggregating and post-translational modification phenomena that can affect the sample.
Protein heterogeneity is a complex property characterized by both transient and permanent forms of modification – which are also subject to individual variations throughout a distinct protein’s lifespan. The resultant range of heterogeneous variability is subsequently vast. It extends far beyond the potential for modification suggested by the underlying amino acids in the polymeric chain alone.
Analyzing these essential protein properties is both challenging and essential. Homo- and heterogeneity of protein samples may be indicative of purity, long-term stability, informational content, physical and mechanical behavior in formulation, and more.
Jordi Labs specializes in the analysis of protein properties using a suite of high technology analytical tools. We utilize gel permeation chromatography (GPC) with multiangle light scattering and high mass accuracy mass spectrometry (MS) to ensure protein homogeneity where purity is of paramount importance.
Analyzing Protein Properties with Jordi Labs
Jordi Labs offers a range of services to assist in the full analysis of your protein properties for a raft of potential application areas. If you would like any more information about our laboratory services, please do not hesitate to contact a member of the Jordi Labs team today.