Physical instability is one of the many obstacles to new drug developments, representing a range of mechanisms that can affect products at practically any stage of production. In protein science, physical instability is primarily represented by aggregation. This is an extremely complex process characterized by multiple distinct mechanisms, resulting in aggregates with dissimilar properties. It is vital that these aggregation states be characterized to ensure optimal stability and efficacy of new and emerging biopharmaceuticals.
Jordi Labs specializes in the analysis of protein aggregation states as a function of both size and molecular weight. Proteins in solution are subject to Brownian motion, and their heterogeneous surfaces and intricate 3D structures can change radically in response to environmental changes. To identify aggregates at extremely low concentrations (<0.01%) and characterize them by size, we use dynamic light scattering (DLS). We can determine the full particle size distribution to aid in a foundational understanding of aggregation state in your colloidal biopharmaceuticals.
Our core competency, however, is in the analysis of protein aggregates by molecular weight determination with light scattering and viscometry. Using advanced triple detection gel permeation chromatography (GPC), we can assist in the development and refinement of biologics for optimal stability in end-use conditions. This is an essential tool for ensuring drug efficacy by detecting and assisting in the characterization of aggregate structures in protein-based pharmaceuticals.
Protein Testing with Jordi Labs
Jordi Labs is one of the world’s leading experts in protein analysis for life sciences and biopharmaceutical applications. We have already applied our unique experience to a range of size and molecular weight studies of protein aggregates to help produce new generations of pharmaceutical products.
If you would like to learn more about testing the size and molecular weight of proteins and protein aggregates, please contact a member of the team.