Liquid Chromatography

High Performance Liquid Chromatography (HPLC)

Jordi Labs offers High Performance Liquid Chromatography (HPLC) services with expertise using normal phase, reverse phase, and Hydrophilic Interaction Chromatography (HILIC) applications.

To quantitate a wide variety of compounds, we offer UltraViolet (UV), Refractive Index (RI), and Evaporative Light Scattering (ELSD) detection modes.

Please find below a list of the chromatographic methods we offer with a brief description of their advantages to help you identify the right technique for your analysis.

Detectors

Ultraviolet (UV) Diode Array Detector (DAD)

• Applicable when the analyte of interest contains rings or double bonds which absorb UV light. Typically, a wavelength from 210-254 nm is selected, and sensitivity of the detector can be in the part per billion (ppb) range.  With DAD, a UV spectrum of a chromatographic peak can be recorded.  This can be helpful when comparing the UV spectrum of a peak from a standard with a peak from a sample for purity analysis.

Evaporative Light Scattering (ELSD)

• Considered a “universal” detector, as all semi- and non-volatile analytes in a sample produce a response.  Sensitivity of the detector can be in the part per million (ppm) range.  This mode offers greater sensitivity than RI detection.

Refractive Index (RI)

• Like ELSD, this is also considered a “universal” detector. RI provides detection of the greatest range of analytes.